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EDTA Treatment of Serum Unmasks Complement-Mediated Prozone Inhibition in Human Leukocyte Antigen Antibody Testing.

Authors
  • Anani, Waseem Q1
  • Zeevi, Adriana1
  • Lunz, John G2
  • 1 From the Department of Pathology, University of Pittsburgh Medical Center, Pittsburgh, PA.
  • 2 From the Department of Pathology, University of Pittsburgh Medical Center, Pittsburgh, PA. [email protected]
Type
Published Article
Journal
American Journal of Clinical Pathology
Publisher
Oxford University Press
Publication Date
Sep 01, 2016
Volume
146
Issue
3
Pages
346–352
Identifiers
DOI: 10.1093/ajcp/aqw116
PMID: 27543980
Source
Medline
Keywords
License
Unknown

Abstract

EDTA serum pretreatment mitigated complement-mediated prozone inhibition and improved accurate HLA antibody detection. The background reactivity and the false-negative rate of the assay appear unchanged.

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