Affordable Access

Publisher Website

Membrane perturbing factor in reticulocyte lysate, which is transiently activated by proteases

Authors
Journal
FEBS Letters
0014-5793
Publisher
Wiley Blackwell (John Wiley & Sons)
Publication Date
Volume
454
Issue
3
Identifiers
DOI: 10.1016/s0014-5793(99)00842-x
Keywords
  • Membrane Topology
  • Protease Treatment: Membrane Degradation
  • Mpl
  • Mature Prolactin
  • Pdi Protein Disulfide Isomerase
  • Ppl
  • Preprolactin: Rl
  • Reticulocyte Lysate
  • Rm
  • Rough Microsomal Membrane
Disciplines
  • Biology
  • Mathematics

Abstract

Abstract Proteases have been used to examine the topology of proteins on various membranes. We reexamined the conditions of protease treatment for rough microsomal membranes and found that proteinase K degraded the lumenal proteins in the presence of reticulocyte lysate. The lysate treated with either heat or N-ethylmaleimide no longer promoted the degradation. The reticulocyte dependent degradation was also observed with papain, trypsin, and elastase. This activity was transiently generated by treating reticulocyte lysate short-term with trypsin. We thus concluded that a membrane perturbing factor(s) must exist in reticulocyte which is transiently activated by protease treatment.

There are no comments yet on this publication. Be the first to share your thoughts.

Statistics

Seen <100 times
0 Comments

More articles like this

An import factor in reticulocyte lysates which sti...

on Acta chemica Scandinavica. Ser... November 1987

Purification of two high molecular weight protease...

on Journal of Biological Chemistr... Jun 15, 1987

Regulation of protein synthesis in rabbit reticulo...

on Proceedings of the National Ac... August 1976
More articles like this..