The research of soursop old leaf methanol extract bioactivity- Annona muricata L., as antibacterial against staphylococcus aureus and Propionibacterium has done. The old leaf used in this research was soursop leaf-Annona muricata L. for fifth and seventh rank from the bud. Each kind of leaves were extracted by using maceration method with methanol as the dissolver. Soursop methanol extract was added with NaCMC (Natrium Carboxy Methyl Cellulose) and diluted at 5%, 10%, 15%, 20% dan 25 b/v concentration. The antibacterium test was done by using agar diffusion method at the MHA (Mueller Hinton Agar) medium. After being incubated for 24 hours, the result showed that the biggest bioactivity result should by 25% concentration, either in soursop old leaf for fifth or seventh rank. The biggest zone of inhibition diameters of soursop old leaf for fifth and seventh rank, ordinally, are 14 mm and 14,5 mm to Staphylococcus aureus, whereas the biggest zone of inhibition diameters to Propionibacterium acnes are 10,5 mm and 12,5 mm. After 48 hours, the zone of inhibition diameters are declined up to 13,5 mm and 14 mm to Staphylococcus aureus, whereas zone of inhibition diameters to Propionibacterium acnes are declined up to 10 mm and 12 mm, are called bacteriostatic.