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Transcription factor AP-2delta regulates the expression of polysialyltransferase ST8SIA2 in chick retina

Authors
  • Li, Xiaodong
  • Persad, Amit R.L.
  • Monckton, Elizabeth A.
  • Godbout, Roseline1, 2, 3
  • 1 Department of Oncology
  • 2 University of Alberta
  • 3 Cross Cancer Institute
Type
Published Article
Journal
FEBS Letters
Publisher
Wiley (John Wiley & Sons)
Publication Date
Jan 01, 2014
Accepted Date
Jan 10, 2014
Volume
588
Issue
5
Pages
770–775
Identifiers
DOI: 10.1016/j.febslet.2014.01.024
Source
Elsevier
Keywords
License
Unknown

Abstract

The AP-2δ transcription factor is restricted to a subset of retinal ganglion cells. Overexpression of AP-2δ in chick retina results in induction of polysialylated neural cell adhesion molecule (PSA-NCAM) accompanied by misrouting and bundling of ganglion cell axons. Two polysialyltransferases, ST8SIA2 and ST8SIA4, are responsible for polysialylation of NCAM. Here, we investigate the mechanism driving the increase in PSA-NCAM observed upon AP-2δ overexpression. We show that ST8SIA2 is induced by AP-2δ overexpression in chick retina. We use chromatin immunoprecipitation and gel shift assays to demonstrate direct interaction between AP-2δ and the ST8SIA2 promoter. We propose that up-regulation of ST8SIA2 upon AP-2δ overexpression in retina increases ectopic polysialylation of NCAM which in turn causes premature bundling of axons and alters axonal response to guidance cues.

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