Affordable Access

deepdyve-link
Publisher Website

Ectopic expression of a phytochrome B gene from Chinese cabbage (Brassica rapa L. ssp. pekinensis) in Arabidopsis thaliana promotes seedling de-etiolation, dwarfing in mature plants, and delayed flowering.

Authors
  • Song, Mei-Fang
  • Zhang, Shu
  • Hou, Pei
  • Shang, Hong-Zhong
  • Gu, Hai-Ke
  • Li, Jing-Juan
  • Xiao, Yang
  • Guo, Lin
  • Su, Liang
  • Gao, Jian-Wei
  • Yang, Jian-Ping
Type
Published Article
Journal
Plant molecular biology
Publication Date
Apr 01, 2015
Volume
87
Issue
6
Pages
633–643
Identifiers
DOI: 10.1007/s11103-015-0302-5
PMID: 25724426
Source
Medline
License
Unknown

Abstract

Phytochrome B (phyB) is an essential red light receptor that predominantly mediates seedling de-etiolation, shade-avoidance response, and flowering time. In this study, we isolate a full-length cDNA of PHYB, designated BrPHYB, from Chinese cabbage (Brassica rapa L. ssp. pekinensis), and we find that BrphyB protein has high amino acid sequence similarity and the closest evolutionary relationship to Arabidopsis thaliana phyB (i.e., AtphyB). Quantitative reverse transcription (RT)-PCR results indicate that the BrPHYB gene is ubiquitously expressed in different tissues under all light conditions. Constitutive expression of the BrPHYB gene in A. thaliana significantly enhances seedling de-etiolation under red- and white-light conditions, and causes dwarf stature in mature plants. Unexpectedly, overexpression of BrPHYB in transgenic A. thaliana resulted in reduced expression of gibberellins biosynthesis genes and delayed flowering under short-day conditions, whereas AtPHYB overexpression caused enhanced expression of FLOWERING LOCUS T and earlier flowering. Our results suggest that BrphyB might play an important role in regulating the development of Chinese cabbage. BrphyB and AtphyB have conserved functions during de-etiolation and vegetative plant growth and divergent functions in the regulation of flowering time.

Report this publication

Statistics

Seen <100 times