Affordable Access

[Ecdysterone induces the activity of multiple forms of acid phosphatase and malate dehydrogenase in fat bodies of Anterahea pernyi pupae].

Authors
  • Kutuzova, N M
  • Filippovich, Iu B
  • Kholodova, Iu D
  • Miladera, K
  • Kindruk, N L
  • Korniets, G V
  • Moroz, N S
Type
Published Article
Journal
Ukrainskiĭ biokhimicheskiĭ zhurnal (1978)
Publication Date
Jan 01, 1991
Volume
63
Issue
3
Pages
41–45
Identifiers
PMID: 1926586
Source
Medline
License
Unknown

Abstract

The method of electrophoresis in PAAG has been used to study activity of multiple forms of acid phosphatase and malate dehydrogenase of the fat body of Anterahea pernyi pupae in norm and affected by ecdysterone. It is shown that an increase of total activity of acid phosphatase and malate dehydrogenase with administration of a steroid hormone is promoted by growth of activity of certain inducible multiple forms of the studied enzymes. Ecdysterone injection considerably elevates activity of the most electrophoretically mobile form of acid phosphatase and provokes new formation of malate dehydrogenase form. Ecdysterone-induced increase of activity of the studied enzyme forms in the fat body of Anterahea pernyi pupae is blocked by actinomycin D, an inhibitor of transcriptional processes, and by puromycin, a translation inhibitor. These data permit concluding that an increase in activity of the mentioned forms of enzymes observed with hormonal induction is a result of their synthesis de novo and is mediated by the DNA-dependent RNA-synthesis.

Report this publication

Statistics

Seen <100 times