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Assembly of recombinant coat protein of sugarcane streak mosaic virus into potyvirus-like particles

National Institute of Science Communication and Information Resource
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  • Biochemistry
  • Biology


Coat protein (CP) gene of sugarcane streak mosaic virus-AP isolate (SCSMV-AP) was expressed in E. coli and recombinant CP (SCSMV-AP rCP) was purified by linear sucrose density gradient centrifugation. Observation of purified SCSMV-AP rCP under electron microscope revealed the presence of potyvirus-like particles (PVLPs). The assembled particles were shown to encapsidate CP gene transcripts by slot-blot hybridization.

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