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Deciphering the role of stress responsive genes in chickpea (Cicer arietinum L.).

Authors
Publication Date
Keywords
  • Chickpea
Disciplines
  • Biology

Abstract

Deciphering the role of stress responsive genes in chickpea (Cicer arietinum L.) Himabindu Kudapa1, Hari C Sharma1, L Krishnamurthy1, Sowmya Adivi1, Peter Vijay1, Andrew D Farmer2, Trushar Shah1, Pooran Gaur1, Rajeev K Varshney1,3* 1International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Hyderabad, India 2National Centre for Genome Resources (NCGR), Santa Fe, NM, USA 3Generation Challenge Programme (GCP), c/o CIMMYT, Mexico DF, Mexico. *E-mail: [email protected] Abstract Chickpea, an important legume crop is seriously challenged by several abiotic and biotic stresses. Understanding the mechanisms that regulate the expression of stress-related genes is a fundamental issue and is necessary for the genetic improvement of chickpea. In separate studies based on in silico expression analysis of Illumina GA and Sanger sequencing data generated from drought and Helicoverpa challenged tissues of contrasting parental genotypes provided differentially expressed stress-responsive genes that are involved in stress signaling pathways. This work presents deciphering the role of in silico identified stress-responsive genes for conferring resistance to drought stress and Helicoverpa infestation. A set of 111 and 55 candidate genes for insect resistance and drought tolerance, respectively, were targeted for experimental validation using quantitative real time-PCR (qRT-PCR). Total RNA of root (drought) and leaf (Helicoverpa) tissues from stressed and unstressed plants were analysed in the context of tolerant/sensitive genotypes. qRT-PCR analysis confirmed in silico predicted expression analysis for a total of 24 genes. This set of genes includes 15 known stress-responsive genes and 9 novel unreported genes. In terms of cross-talking between genes under these two stresses three genes encoding for mannitol dehydrogenase, NADPH quinine oxidoreductase and cytochrome P-450 showed differential expression. Emerging evidence suggests that stress si

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