Affordable Access

Publisher Website

Active Suppressor tRNAs with a Double Helix between the D- and T-loops

Authors
Journal
Journal of Molecular Biology
0022-2836
Publisher
Elsevier
Publication Date
Volume
373
Issue
2
Identifiers
DOI: 10.1016/j.jmb.2007.07.058
Keywords
  • L-Shape
  • Molecular Modelling
  • Selectionin Vivo
  • T-Loop
  • Trna

Abstract

Abstract One of the most conserved elements of the tRNA structure is the reverse-Hoogsteen base-pair T54–A58 in the T-loop, which plays a major role in the maintenance of the standard L-shape conformation. Here, we present the results of in vivo selection of 51 active suppressor tRNA clones, none of which contains base-pair T54–A58. In 49 clones, we found two regions in the D and T-loops that are complementary to each other. This finding suggests the existence of an inter-loop double helix consisting of three base-pairs, which could have the same role as base-pair T54–A58 in the fixation of the juxtaposition of the two helical domains within the L-shape. From this point of view, the appearance of the inter-loop double helix represents a compensatory effect for the absence of base-pair T54–A58. The results shed new light on the role of different elements of the tRNA structure in the formation of the standard L-shape conformation and on the possibility of synonymous replacements of one arrangement by another in functional RNA molecules.

There are no comments yet on this publication. Be the first to share your thoughts.

Statistics

Seen <100 times
0 Comments

More articles like this

Active suppressor tRNAs with a double helix betwee...

on Journal of Molecular Biology Oct 19, 2007

Helix 69 in 23S rRNA modulates decoding by wild ty...

on Molecular genetics and genomic... October 2009

The distance between the anticodon loops of two tR...

on Journal of Molecular Biology Aug 25, 1979

The distance between the anticodon loops of two tR...

on Journal of Molecular Biology Jan 01, 1979
More articles like this..