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A dynamic combinatorial screen for novel imine reductase activity

Authors
Publication Date
Keywords
  • Acetobacterium Woodii (Dynamic Combinatorial Screen For Novel Imine Reductase Activity)
  • Butylidine Aniline Benzylidine Imine Reductase Acetobacterium Woodii Amine
Disciplines
  • Biology
  • Mathematics

Abstract

New imine reductase activity has been discovered in the anaerobic bacterium Acetobacterium woodii by screening a dynamic combinatorial library of virtual imine substrates, using a biphasic water-tetradecane solvent system. Benzylidine aniline and butylidine aniline were reduced to the corresponding amines by caffeate-induced cells, whereas uninduced cells reduced butylidine aniline only. The redns. were detected despite side reactions that consumed some of the starting materials. The new screen can now be extended to discover synthetically useful imine reductases and enzymes that catalyze reactions for which biocatalytic equiv. of the chem. reactions have not yet been discovered. [on SciFinder (R)]

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