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Ebola virus secreted glycoprotein decreases the anti-viral immunity of macrophages in early inflammatory responses.

Authors
  • Bradley, Jillian H1
  • Harrison, Ametria2
  • Corey, Ashley1
  • Gentry, Nathan1
  • Gregg, Randal K3
  • 1 Division of Biomedical Sciences, Magnolia Research Center, Department of Microbiology and Immunology, Edward Via College of Osteopathic Medicine - Carolinas Campus, Spartanburg, SC 29303, United States. , (United States)
  • 2 Department of Biology, Chemistry and Physics, Converse College, Spartanburg, SC 29301, United States. , (United States)
  • 3 Division of Biomedical Sciences, Magnolia Research Center, Department of Microbiology and Immunology, Edward Via College of Osteopathic Medicine - Carolinas Campus, Spartanburg, SC 29303, United States. Electronic address: [email protected] , (United States)
Type
Published Article
Journal
Cellular Immunology
Publisher
Elsevier
Publication Date
Feb 01, 2018
Volume
324
Pages
24–32
Identifiers
DOI: 10.1016/j.cellimm.2017.11.009
PMID: 29195741
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

During Ebola virus (EBOV) infection, secreted glycoprotein (sGP) is found in large quantities in the serum of both patients and infected animal models. It is thought to serve as a decoy for anti-EBOV antibodies. Using an in vitro model incorporating treatment of non-infected human THP-1 macrophages with recombinant EBOV sGP, this study sought to examine the impact of sGP upon key macrophage functions. Macrophage polarization and phagocytic capacity of activated macrophages were found to be unaltered by sGP treatment. However, treatment with sGP inhibited macrophage production of the pro-inflammatory cytokines TNFα and IL-6 while the yield of anti-inflammatory cytokine, IL-10, remained intact. Interestingly, the migratory ability of macrophages was also diminished by sGP, potentially due to a decrease in expression of CD11b, a vital macrophage integrin. Thus, EBOV sGP may operate to diminish functional contributions of non-infected macrophages to increase the potential viral dissemination. Copyright © 2017 Elsevier Inc. All rights reserved.

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