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Application of the polymerase chain reaction to the detection of African horse sickness viruses

Authors
Journal
Journal of Virological Methods
0166-0934
Publisher
Elsevier
Publication Date
Volume
53
Issue
1
Identifiers
DOI: 10.1016/0166-0934(94)00175-g
Keywords
  • African Horse Sickness Viras
  • Coupled Rt-Pcr
  • Elisa

Abstract

Abstract The development of a coupled reverse transcriptase-polymerase chain reaction assay (RT-PCR) is described for the detection of African horse sickness virus (AHSV) double-stranded RNA. Genome segments 7 and 10 were chosen as target templates for primers selected for use in the RT-PCR. Using these AHSV-specific primers all 9 serotypes were detectable. The sensitivity and specificity of the RT-PCR results were compared to those obtained by competition ELISA.

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