Graphical abstract (+)-3,5,7-Trihydroxy-3-[3′-hydroxy-2′,4′-dimethoxy-5-(3-methyl-2-butenyl)]-phenyl-(3R)-4H-1-benzopyran-4-one (1) and (−)-3-hydroxy-8,9-methylenedioxy-(6aR,11aS)-pterocarpan (2), were isolated from the methanolic extract of Sophora mollis subsp. griffithii. Two known compounds, β-sitosterol (3) and 19βH-lupeol-methyl-ether (4), obtained for the first time from this plant. Compounds 1, 3 and 4 were studied for their effects on immune cells, and only 1 was found to be substantially active. Highlights ► Sophora mollis is a small shrub with yellow flowers. ► Two new compounds 1–2 were isolated from the methanolic extract of Sophora mollis. ► β-Sitosterol and 19βH-lupeol-methyl-ether were isolated for the first time from this plant. ► The isolated compounds were evaluated for their immunomodulatory potential. ► Compound 1 exhibited significant immunomodulatory activity.