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Nucleic acid amplification-based techniques for pathogen detection and identification

Authors
Journal
Infection Genetics and Evolution
1567-1348
Publisher
Elsevier
Publication Date
Volume
6
Issue
1
Identifiers
DOI: 10.1016/j.meegid.2005.08.004
Keywords
  • Nucleic Acid
  • Amplification
  • Detection
  • Real-Time Pcr
  • Pcr
  • Nasba
  • Biosensor
  • Lcr
Disciplines
  • Chemistry
  • Medicine

Abstract

Abstract Nucleic acid amplification techniques have revolutionised diagnostic and research industries. Current technologies that allow the detection of amplification in real-time are fast becoming industry standards, particularly in a diagnostic context. In this review, we describe and explore the application of numerous real-time detection chemistries and amplification techniques for pathogen detection and identification, including the polymerase chain reaction, nucleic acid sequence-based amplification, strand displacement amplification and the ligase chain reaction. The emergence of newer technologies, such as lab-on-a-chip devices and photo-cleavable linkers, is also discussed.

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