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Acetylcholine enzyme sensor for determining methamidophos insecticide:Evaluation of some genetically modified acetylcholinesterases from Drosophila melanogaster

Authors
Journal
Analytica Chimica Acta
0003-2670
Publisher
Elsevier
Publication Date
Volume
434
Issue
1
Identifiers
DOI: 10.1016/s0003-2670(01)00795-4
Keywords
  • Screen-Printed Electrodes
  • Methamidophos
  • Recombinant Acetylcholinesterases
  • Inhibition Constant (Ki)
Disciplines
  • Biology

Abstract

Abstract A sensitive screen-printed amperometric sensor suitable for rapid determination of the concentration of the insecticide methamidophos was developed. It was based on the principle of inhibition of acetylcholinesterase (AChE) activity. The first part of the study was focused on the screening of several genetically modified AChEs in order to select the most sensitive enzyme towards the methamidophos. Values for the bimolecular constant k i were also determined. In a second part of the study, we compared the lowest detectable methamidophos concentrations using different immobilised AChEs. The lowest detectable concentration in a standard solution was 1.4 ppb for the AChE(Dros)-B03 mutant compared to 4.8 ppb for the wild type AChE and 53 ppb for the electric eel source.

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