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A technique for studying chemotaxis of leucocytes in well-defined chemotactic fields

Authors
Journal
Experimental Cell Research
0014-4827
Publisher
Elsevier
Publication Date
Volume
79
Issue
2
Identifiers
DOI: 10.1016/0014-4827(73)90457-6
Disciplines
  • Biology
  • Chemistry

Abstract

Abstract A new technique for observing the chemotaxis of leucocytes in well-defined chemotactic fields has been developed. This technique is also applicable to other studies in cell physiology in which an efficient method of generating known chemical concentration gradients on microscope slides is needed. This study reports the range of concentrations and gradients of cyclic adenosine 3′, 5′-monophosphate which will result in chemotactic ameboid motion of human polymorphonuclear granulocytes. We have identified a threshold gradient and a range of concentration near the solubility limit in which chemotaxis is impaired.

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