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Isolation of the minicircular DNA ofEscherichia coli15 as a DNA-Protein complex

Authors
Journal
Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis
0005-2787
Publisher
Elsevier
Publication Date
Volume
281
Issue
3
Identifiers
DOI: 10.1016/0005-2787(72)90464-9
Disciplines
  • Biology

Abstract

Abstract 1. 1. The minicircular DNA of Escherichia coli 15 can be isolated as a DNA-protein complex (form I★) by sucrose gradient centrifugation in low salt (0.1 M NaCl). 2. 2. Form I★ can be converted into the non-complexed supercoiled form I in high salt (1 M NaCl) or by incubation with Pronase. It differs from the DNA-protein complexes of other plasmids by not undergoing a conversion to the open circular form II under conditions known to affect protein structure. 3. 3. Synthesis of the protein component of form I★ is inhibited by chloramphenicol. After a prolonged treatment with chloramphenicol the replication of the minicircular DNA continues but the newly replicated DNA is found almost exclusively in form I.

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