Affordable Access

Publisher Website

Nitric oxide induces osteoblast apoptosis through the de novo synthesis of Bax protein

Journal of Orthopaedic Research
Wiley Blackwell (John Wiley & Sons)
Publication Date
DOI: 10.1016/s0736-0266(01)00086-9
  • Biology
  • Chemistry
  • Design


Abstract Nitric oxide (NO) plays a crucial role in the physiological and pathophysiological regulations of osteoblast functions. This study is designed to evaluate the toxic effects of NO released by sodium nitroprusside (SNP), an NO donor, on neonatal Wistar rat calvarial osteoblasts from the analyses of cell viability, alkaline phosphatase (ALP) activity, cell morphology, apoptotic cells, terminal deoxynucleotidyl transferase-mediated dUTP nick end-label (TUNEL) assay, DNA ladder, and immunocytochemistry and Western blot for proapoptotic Bax protein. SNP increased the levels of nitrite, an oxidative product of NO, in the culture medium of osteoblasts in concentration- and time-dependent manners, and altered cell morphologies to round and shrinkage shapes. Administration of osteoblasts with SNP resulted in concentration- and time-dependent decreases of cell viability and ALP activity. Analysis of apoptotic cells revealed that SNP increased the percentages of osteoblasts processing apoptosis. Analyses of TUNEL and DNA ladder showed that SNP caused DNA fragmentation. Pretreatment with cycloheximide, an inhibitor of protein synthesis, partially blocked SNP-induced osteoblast apoptosis. Imunocytochemical and immunoblotting analyses revealed that SNP increased Bax protein in osteoblasts. This study suggests that SNP could increase the levels of NO in osteoblasts, and cause osteoblast apoptosis possibly through the de novo synthesis of proapoptotic Bax protein.

There are no comments yet on this publication. Be the first to share your thoughts.


Seen <100 times

More articles like this

Nitric oxide induces osteoblast apoptosis through...

on Journal of orthopaedic researc... March 2002

Nitric oxide induces osteoblast apoptosis through...

on Annals of the New York Academy... May 2005

Phenylarsine oxide induces apoptosis in Bax- and B...

on Clinical Cancer Research Jan 01, 2012

Suppression of nitric oxide-induced apoptosis by N...

on Molecular Carcinogenesis June 1997
More articles like this..