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Conformational changes at the peptidyl transferase center of antibiotic resistant mutants ofSaccharomyces cerevisiae

Authors
Journal
Biochemical and Biophysical Research Communications
0006-291X
Publisher
Elsevier
Publication Date
Volume
94
Issue
3
Identifiers
DOI: 10.1016/0006-291x(80)91305-4
Disciplines
  • Biology

Abstract

Abstract Ribosomes from Saccharomyces cerevisiae wild type (Y166) and trichodermin (TR1) and narciclasine (NAR2b) resistant strains were affinity labeled with p-nitrophenylcarbamyl-( 3H)Phe-tRNA. Resistant ribosomes were able to bind covalently two to five times more aminoacyl-tRNA derivative than control. The labeling pattern of the individual ribosomal proteins was also altered in the two mutants. These results evidence a change in the conformation of the peptidyl transferase center that brings the 3′ end of the aminoacyl-tRNA closer to several ribosomal proteins.

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