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A rapid ELISA for measurement of antibodies to nucliec acid antigens using UV-treated polystyrene microplates

Authors
Journal
Journal of Immunological Methods
0022-1759
Publisher
Elsevier
Publication Date
Volume
90
Issue
1
Identifiers
DOI: 10.1016/0022-1759(86)90390-x
Keywords
  • Elisa
  • Anti-Dna Antibodies
  • Systemic Lupus Erythematosus
  • Uv Irradiation

Abstract

Abstract Pretreatment of polystyrene microplate wells with certain doses of UV light enhances their capacity for binding to single-stranded DNA, double stranded DNA and various synthetic polynucleotides. The use of UV-irradiated plates to immobilize nucleic acid antigens provides a simple, rapid, and specific ELISA for measuring anti-nucleic acid antibodies. The assay is at least as sensitive as the more complex method of precoating plates with poly( L-lysine). It is useful for detection of anti-DNA antibodies in sera of systemic lupus erythematosus patients, as well as in culture fluids of murine and human anti-DNA-secreting hybridomas.

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