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Highly-resistant E. coli as a Common Cause of Paediatric Diarrhoea in India

Journal of Health Population and Nutrition
Bangladesh Journals Online
Publication Date
  • Letter-To-The-Editor
  • Biology
  • Chemistry
  • Medicine


©INTERNATIONAL CENTRE FOR DIARRHOEAL DISEASE RESEARCH, BANGLADESH J HEALTH POPUL NUTR 2013 Sep;31(3):409-412 ISSN 1606-0997 | $ 5.00+0.20 Correspondence: (Reprints are not available from the author) Dr. Prabhav Aggarwal E-49, Sector-55, NOIDA Uttar Pradesh 201 301 India Email: [email protected] riod with acute diarrhoea (with or without visible blood/mucus) of duration less than 14 days were included in the study. In total, stool samples were collected from 347 children in clean plastic con- tainers and transported to microbiology laboratory within two hours after collection. In case of delay of more than two hours, samples were transported in Cary-Blair medium/buffered glycerol saline, or stored at 4 °C. Wet mounts were examined for pres- ence of pus cells, RBC, and parasitic ova/cysts or trophozoites. Stool samples were cultured on Mac- Conkey agar, Xylose desoxycholate agar (XLD), Salmonella-Shigella agar, and bile salt agar (BSA). The stool samples were also inoculated in Selenite F broth and alkaline peptone water (APW) for en- richment. The plates were incubated at 37 °C for 18-24 hours. The organisms were identified on the basis of colony characteristics and biochemical re- actions. E. coli isolates were subjected to serotyping by the slide agglutination test (Denka Seiken Co., Ltd., Tokyo, Japan). Bacterial enteropathogens were subjected to anti- microbial susceptibility testing, using disc diffusion method (3) against a wide range of antimicrobial agents and phenotypic confirmatory test for ESBL production according to the CLSI guidelines (2011) (3). ATCC25922 was used as the control strain. De- creased susceptibility to ceftazidime (zone diameter ≤22 mm) and/or cefotaxime (zone diameter ≤27 mm) encountered was used as initial screening test for ESBL production. These isolates were subjected to the phenotypic confirmatory test (3) by the combination disc method, utilizing (i) ceftazidime (30 µg) and ceftazidime/clavulanic acid (30 µg+10 µg

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