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Hapten synthesis and antibody production for the development of a melamine immunoassay

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  • The Incorporation Of Melamine Into Food Products Is Banned But Its Misuse Has Been Widely Reported I
  • The Development Of A Rapid Screening Immunoassay For Monitoring Of The Substance Is An Urgent Requir
  • Two Haptens Of Melamine Were Synthesized By Introducing Spacer Arms Of Different Lengths And Structu
  • 6-Aminocaproic Acid And 3-Mercaptopropionic Acid Were Reacted With 2-Chloro-4
  • 6-Diamino-1
  • 3
  • 5-Triazine (Caat) To Produce Hapten 1[3-(4
  • 6-Diamino-1
  • 6-Dihydro-1
  • 3
  • 5-Triazin-2-Ylamino) Hexanoic Acid] And Hapten 2[3-(4
  • 6-Diamino-1
  • 6-Dihydro-1
  • 3
  • 5-Triazin-2-Ylthio) Propanoic Acid]
  • Respectively
  • The Molecular Structures Of The Two Haptens Were Identified By I H Nuclear Magnetic Resonance Spectr
  • Mass Spectrometry And Infrared Spectrometry
  • An Immunogen Was Prepared By Coupling Hapten 1 To Bovine Serum Albumin (Bsa)
  • Two Plate Coating Antigens Were Prepared By Coupling Both Haptens To Egg Ovalbumin (Ova)
  • A Competitiveindirect Enzyme-Linked Immunosorbent Assay (Cielisa) Was Developed To Evaluate Homogene
  • The Results Showed That Polyclonal Antibodies With High Titers Were Obtained
  • And The Heterogeneous Immunoassay Format Demonstrated A Better Performance With An Ic50 Of 70
  • 6 Ng Ml(-1)
  • A Lod Of 2
  • 6 Ng Ml(-1) And A Loq Of 7
  • 6 Ng Ml(-1)
  • Except For Cyromazine
  • No Obvious Cross-Reactivity To Common Compounds Was Found
  • The Data Showed That The Hapten Synthesis Was Successful And The Resultant Antisera Could Be Used In
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  • V
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  • Biology
  • Medicine


1 Ludwig Eichinger and Francisco Rivero (eds.), Dictyostelium discoideum Protocols, Methods in Molecular Biology 983, DOI 10.1007/978-1-62703-302-2_1, © Springer Science+Business Media, LLC 2013 Chapter 1 The Amoebozoa Christina Schilde and Pauline Schaap Abstract The model organism Dictyostelium discoideum is a member of the Amoebozoa, one of the six major divisions of eukaryotes. Amoebozoa comprise a wide variety of amoeboid and fl agellate organisms with single cells measuring from 5 μ m to several meters across. They have adopted many different life styles and sexual behaviors and can live in all but the most extreme environments. This chapter provides an overview of Amoebozoan diversity and compares roads towards multicellularity within the Amoebozoa with inven- tions of multicellularity in other protist divisions. The chapter closes with a scenario for the evolution of Dictyostelid multicellularity from an Amoebozoan stress response. Key words Amoebozoa , Protista , Aggregative multicellularity , Encystation , Sporulation , Morphogenesis , Cyclic AMP signaling , Phylogeny The Dictyostelids have fascinated biologists for over 150 years with their ability to assemble up to a million amoebas into a tactile migrating organism, which, after seeking out a site for spore dis- persal, transforms into a well-balanced fruiting structure. The development of a range of molecular genetic and cell biological procedures for the species Dictyostelium discoideum over the past 30 years has established this species as an important model organ- ism for the study of fundamental cell biological and developmental processes ( 1 ) . More recently, the evolution of social behavior and the study of genes associated with human diseases and bacterial infections have been added to the repertoire of research questions that can be addressed in Dictyostelia ( 2 ) . With putative applications of research in mind,

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