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Development of a simple, rapid and validated square wave voltametric method for determination of promethazine in raw material and pharmaceutical formulation using DNA modified multiwall carbon nanotube paste electrode

Authors
Journal
Sensors and Actuators B Chemical
0925-4005
Publisher
Elsevier
Volume
177
Identifiers
DOI: 10.1016/j.snb.2012.11.005
Keywords
  • Biosensor
  • Multiwall Carbon Nanotubes
  • Dna
  • Electrochemical Method
  • Promethazine
  • Pharmaceutical Formulation
Disciplines
  • Pharmacology

Abstract

Abstract A simple voltammetric method based on a composite biosensor MWCN/SiO2/Al2O3/Nb2O5/DNA (MWCN/SiAlNb/DNA) was developed and validated for the determination of promethazine (PHZ) in raw material and pharmaceutical formulation. The best results were obtained by using square wave voltammetry under measurements at 0.30molL−1 phosphate buffer pH 7.0 and DNA immobilization onto SiAlNb surface using 0.8mgmL−1 concentration. Validation parameters such as: selectivity, linearity, precision, accuracy, and stability presented results within the acceptable range. The sensitivity of sensor was found to be 0.210μAμmol−1L. Therefore, the developed method was successfully applied for the PHZ determination in real samples of pharmaceutical formulations and can be used for routine quality control analysis of bulk pharmaceutical and pharmaceutical formulations containing PHZ.

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