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Membrane chromatography for the purification of laccase from the supernatant ofPleurotus sapidus

Authors
Journal
Biochemical Engineering Journal
1369-703X
Publisher
Elsevier
Publication Date
Volume
70
Identifiers
DOI: 10.1016/j.bej.2012.11.003
Keywords
  • Membrane Chromatography
  • Membrane Adsorption
  • Downstream Processing
  • Laccase
  • Pleurotus Sapidus
Disciplines
  • Biology

Abstract

Abstract Extracellular laccase from the fermentation broth of Pleurotus sapidus was purified using membrane chromatography. Five laccase iso-enzymes produced in submerged cultures were purified; the enzymes had isoelectric points (pI) ranging from 3.3 to 4.7 and a calculated molecular weight of 57.4kDa. The fermentation broth had a low product titre of 80μg/l. Hydrophobic interaction (HIC) and ion exchange (IEX) membranes chromatography modules were used in an ÄKTApurifier 100 liquid chromatography system for the dynamic experiments. The pre-treatment of the fermentation broth was not required. The enzyme activity was retained, and enrichment up to 15-fold was observed via activity assays and SDS-Page analysis.

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