Aflatoxin contamination is a common occurrence in corn-based agricultural commodities such as grains andoilseed crops that are normally stored in warm moist places. Since aflatoxins are highly toxic and carcinogenic, thisstudy was undertaken to determine the use of ammoniation procedures to destroy aflatoxin in contaminated maizesamples. Strains of Aspergillus parasiticus were used to contaminate maize samples collected from market centersin Eldoret Municipality. They were then decontaminated by ammoniation procedure under normal temperatureand pressure. High performance liquid chromatography was used for analysis of aflatoxin content in maize cropsamples. The results revealed that ammoniation procedure can destroy over 90% of the aflatoxin in maize and maybe considered economically viable especially where alternative foodstuffs are not available or affordable.