Affordable Access

Publisher Website

Genetic alterations and protein expression of KIT and PDGFRA in serous ovarian carcinoma

Authors
Journal
British Journal of Cancer
0007-0920
Publisher
Nature Publishing Group
Publication Date
Identifiers
DOI: 10.1038/sj.bjc.6602252
Keywords
  • Molecular And Cellular Pathology
Disciplines
  • Biology
  • Chemistry
  • Ecology

Abstract

Genetic alterations and protein expression of KIT and PDGFRA in serous ovarian carcinoma H Lassus1, H Sihto2, A Leminen1, S Nordling3, H Joensuu2, NN Nupponen2 and R Butzow*,1,3 1Department of Obstetrics and Gynecology, Helsinki University Central Hospital, Haartmaninkatu 2, Helsinki 00290, Finland; 2Department of Oncology, Helsinki University Central Hospital, Haartmaninkatu 4, Helsinki 00290, Finland; 3Department of Pathology, University of Helsinki, PO Box 21 (Haartmaninkatu 3), Helsinki 00014, Finland KIT and PDGFRA are receptor tyrosine kinases that can be specifically inactivated by small-molecule tyrosine kinase inhibitors, notably imatinib mesylate. In ovarian carcinoma, expression of KIT and PDGFRA protein has been documented, but the frequency and the molecular background of expression are poorly known. We analysed the expression of KIT and PDGFRA by immunohistochemistry in 522 serous ovarian carcinomas, and mutations of KIT and PDGFRA by denaturing high-performance liquid chromatographyin 125 and 187 serous ovarian carcinomas, respectively. No mutations of KIT or PDGFRA were detected. KIT expression was detected in 12% of carcinomas: low expression in 10% and high expression in 2% of cases. Using normal serous epithelium as a reference, decreased PDGFRA expression was detected in 12% and increased expression in 13% of carcinomas. Both KIT and PDGFRA expression were associated with high tumour grade, high proliferation index and poor patient outcome. By fluorescence in situ hybridisation, no KIT amplification was found in carcinomas with high KIT expression, but two cases showed a relative gain of chromosome 4. In conclusion, no mutations of KIT or PDGFRA were found, but a subset of serous ovarian carcinoma showed overexpression of the proteins, which was associated with aggressive tumour characteristics. British Journal of Cancer (2004) 91, 2048–2055. doi:10.1038/sj.bjc.6602252 www.bjcancer.com Published online 7 December 2004 & 2004 Cancer Research UK Keywords: ovarian neopl

There are no comments yet on this publication. Be the first to share your thoughts.

Statistics

Seen <100 times
0 Comments

More articles like this

Genetic alterations and protein expression of KIT...

on British Journal of Cancer Dec 13, 2004

Protein expression and gene mutation status of KIT...

on Histology and histopathology March 2012

The impact of c-kit and ki-67 expression on patien...

on International journal of gynec... July 2005

Genetic alterations of serous borderline tumors of...

on Cancer Genetics and Cytogeneti... June 2006
More articles like this..