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Možnosti využití signálního genu pro zeleně fluoreskující protein k identifikaci a selekci transformantů lnu

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  • Zeleně Fluoreskující Protein
  • Transformace Rostlin
  • Selekce
  • Fluorescence
  • Genová Exprese
  • Green Fluorescent Protein
  • Plant Transformation
  • Selection
  • Fluorescence
  • Gene Expression
  • Biology


An aim of the thesis was to verify possibilites of in vitro identification and selection of transformed flax tissues and plants based on the fluorescence assessment of objects transformed with a construct bearing the gfp gene coding for green fluorescent protein (GFP). Effect of plant genotype, explant type and modifications of the transformation protocol on fluorescence was evaluated too, as well as the possibility of use for selection of transgenic individuals.

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