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Murine epidermal lipoxygenase (Aloxe) encodes a 12-lipoxygenase isoform1 The sequence data reported in this paper have been submitted to the EMBL/GenBank Data Libraries under the accession number X99252.1

Authors
Journal
FEBS Letters
0014-5793
Publisher
Wiley Blackwell (John Wiley & Sons)
Publication Date
Volume
402
Identifiers
DOI: 10.1016/s0014-5793(96)01517-7
Keywords
  • Epidermis
  • Lipoxygenase
  • Cdna Cloning
  • Functional Expression
  • Chromosomal Localization
Disciplines
  • Biology

Abstract

Abstract Using a combination of conventional screening procedures and polymerase chain reaction cloning, we have isolated a cDNA encoding an epidermis-type 12-lipoxygenase ( e12-lipoxygenase) from mouse epidermis. The open reading frame corresponds to a protein of 662 amino acids and was found to be 99.8% identical to the ORF of an epidermal lipoxygenase gene Aloxe, described recently [Van Dijk et al. (1995) Biochim. Biophys. Acta 1259, 4–8]. When expressed in human embryonic kidney cells the recombinant protein could be shown to synthesize 12( S)-HETE from arachidonic acid. By fluorescence in situ hybridization the e12-lipoxygenase gene was localized to chromosome band 11 B1–B3.

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