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Selective interactions of concanavalin A at lipid membranes on the surface of an optical fiber

Authors
Journal
Talanta
0039-9140
Publisher
Elsevier
Publication Date
Volume
37
Issue
8
Identifiers
DOI: 10.1016/0039-9140(90)80122-v

Abstract

Abstract An optical configuration was developed for sampling fluorescence coupled into an optical fiber from evanescent wave excitation of fluorescent materials at a lipid membrane on a quartz fiber surface. Selective interactions of pyrene-labelled concanavalin A located on a phosphatidyl choline—cholesterol lipid membrane with fluorescein isothiocyanate-labelled dextran in bulk aqueous solution were monitored by the intrinsic fluorescence sensing configuration. Monosialoganglioside, G M1, was employed as a receptor in a phospholipid membrane on an optical fiber for selective measurement of pyrene-labelled concanavalin A in solution. Quantitative measurement was hindered by non-selective adsorption of cancanavalin A, but the potential for use of a lipid membrane in a fluorometric biosensor was established.

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