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Cyclin E2 is required for embryogenesis inXenopus laevis

Authors
Journal
Developmental Biology
0012-1606
Publisher
Elsevier
Publication Date
Volume
310
Issue
2
Identifiers
DOI: 10.1016/j.ydbio.2007.08.005
Keywords
  • Cell Cycle
  • Cell Proliferation
  • Cyclin E1
  • Cyclin E2
  • Differentiation
  • Embryogenesis
  • Maternal Zygotic Transition
  • Xenopus Laevis

Abstract

Abstract In mammalian cells, E-type cyclins (E1 and E2) are generally believed to be required for entry into S phase. However, in mice, cyclin E is largely dispensable for normal embryogenesis. Moreover, Drosophila cyclin E plays a critical role in cell fate determination in neural lineages independently of proliferation. Thus, the functions of cyclin E, particularly during early development, remain elusive. Here, we investigated the requirement for E-type cyclins during Xenopus embryogenesis. Although cyclin E1 has been reported as a maternal cyclin, inhibition of its translation in the embryo caused no serious defects. We isolated a Xenopus homologue of human cyclin E2, which was zygotically expressed. Sufficient inhibition of its expression led to death at late gastrula, while partial inhibition allowed survival. These observations indicate distinct roles for Xenopus cyclins E1 and E2, and an absolute requirement of cyclin E2 for Xenopus embryogenesis.

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