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Abundance and biomass of microzooplankton at station CD61_195#5

Publication Date
DOI: 10.1594/pangaea.197565
  • 61
  • 195#5
  • Biogeochemical Ocean Flux Study
  • Bofs
  • Cd61
  • Cd61_195#5
  • Charles Darwin
  • Ctd/Rosette
  • Jgofs
  • Joint Global Ocean Flux Study
  • Microzooplankton
  • Microzooplankton
  • Biomass As Carbon
  • Optical Microscopy
  • Earth Science
  • Geography


Microzooplankton Introduction This document describes the methodology used to derive the data contained in the tables MICMASS and MICGRAZ which include a subset of the bottle data (discrete samples) assembled from the BOFS Atlantic cruises. Each parameter has been documented for sampling and analytical methodologies from information supplied by the analysts; information was also supplied by Research Vessel Services (RVS). Acquisition, Processing and Editing Water bottle samples were obtained either from the General Oceanics Rosette Multisampler (fitted with twelve 10-litre Go-Flo or Niskin water bottles), which was attached to a Neil Brown Mk 3B CTD, or from 7-litre Niskin water bottles or 30-litre Go-Flos, lowered from the electric hydrographic winch. Individual datasets of water bottle data were largely worked up post-cruise by the responsible scientist and submitted to BODC for loading to the database. Datasets were uniquely identified and cross-referenced, using time and depth as the primary linking keys. Quality control was primarily the responsibility of the data originator. However, any errors detected by database users or BODC have been corrected. Microzooplankton biomass Analysis was by microscopy. The following samples were taken for analysis : One litre of unfiltered water was fixed in acid Lugols for the determination of microzooplankton numbers and biomass. On Charles Darwin cruise 46, this was further subsampled, with fractions of the sample being preserved in 2% glutaraldehyde for the determination of nanoplankton abundance or in 2% buffered formaldehyde to determine the proportions of plastidic and non-plastidic ciliates. 20-50ml samples were fixed in glutaraldehyde, stained with DAPI and proflavin and filtered onto nuclepores for the differentiation between photosynthetic and heterotrophic flagellates by epifluorescence microscopy and the determination of their numbers and biomass. Apstein net hauls (20µm mesh) were taken every 1-2 days through the surfac

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