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Aqueous size-exclusion chromatography of humic acids on a sephadex gel column with diluted phosphate buffers as eluents

Authors
Journal
Analytica Chimica Acta
0003-2670
Publisher
Elsevier
Publication Date
Volume
193
Identifiers
DOI: 10.1016/s0003-2670(00)86154-1

Abstract

Abstract The elution behavior of humic acids on a Sephadex gel column is senstive to the composition, concentration, and pH of the eluent. the concentrations of the eluent in the literature are too high to obtain the correct molecular size distribution of humic acids. By reducing the concentration of phosphate buffer eluents to about a hundredth of conventional concentrations, the correct distribution is obtained. In the proposed method, 1 ml of 0.1% sample solution is introduced into a Sephadex G-50 column (2.2-cm diameter, 55 cm long) and humic acids are eluted with a 10 −3 M phosphate buffer solution (pH 7–9) at a flow rate of 1 ml min −1.

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