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Study of molar response of dextrans in electrochemical detection

Authors
Journal
Journal of Chromatography A
0021-9673
Publisher
Elsevier
Publication Date
Volume
1149
Issue
1
Identifiers
DOI: 10.1016/j.chroma.2006.11.086
Keywords
  • Dextrans
  • Oligosaccharide
  • Anion-Exchange Chromatography
  • Pulsed Amperometric Detection
  • Capillary Zone Electrophoresis
Disciplines
  • Chemistry

Abstract

Abstract In this work, a methodological approach is reported, aimed at assessing the electrochemical response of some model gluco-oligosaccharides (dextrans). Such strategy is based on the complementary use of both anion-exchange chromatography with pulsed amperometric detection (HPAEC–PAD) and capillary zone electrophoresis coupled with UV detection (CZE–UV). Unlike HPAEC–PAD, CZE–UV required derivatization with a chromophoric dye (i.e., 8-aminonaphtalene-1,3,6-trisulphonic acid, ANTS) to enhance UV response and separation selectivity. From the comparison between chromophore response and PAD signal, the reliability of HPAEC–PAD for quantitative evaluation of dextran mixtures containing mainly oligomers with polymerization degree (DP) up to 18 could be proved, due to the fairly constant molar response. For higher DPs (up to 41), a maximum in the trend of the molar responses was observed followed by a steep decrease for DPs higher than about 30–35; indeed, an underestimation of weight-average molecular weight of dextran mixtures containing such oligomers was noticed.

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