Affordable Access

Lysine biosynthesis in Saccharomyces. Conversion of α-aminoadipate into α-aminoadipic δ-semialdehyde

Publication Date
  • Articles


The reduction of α-aminoadipate to α-aminoadipic δ-semialdehyde by a cell-free extract of Saccharomyces is shown to be a three-step process. First the amino acid reacts with ATP to form an adenylyl derivative. Then the adenylyl derivative of α-aminoadipate is reduced in the presence of NADPH. In the third step the reduced adenylyl derivative of the amino acid is cleaved to form α-aminoadipic δ-semialdehyde. The presence of Mg2+ is necessary for the first and second steps. The third step does not need any cofactors. The product of the first step was isolated by chromatography after incubating the cell-free extract of Saccharomyces with α-aminoadipate, ATP and Mg2+. The isolated product was identified as an adenylyl derivative of α-aminoadipate and could be converted into α-aminoadipic δ-semialdehyde under the stated experimental conditions. The product of the second step was too unstable to be identified.

There are no comments yet on this publication. Be the first to share your thoughts.


Seen <100 times