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Echinococcus multilocularis primary cells: Improved isolation, small-scale cultivation and RNA interference

Authors
Journal
Molecular and Biochemical Parasitology
0166-6851
Publisher
Elsevier
Volume
174
Issue
1
Identifiers
DOI: 10.1016/j.molbiopara.2010.07.001
Keywords
  • Echinococcus
  • Multilocularis
  • Rnai
  • Interference
  • In Vitro Culture
  • Parasite
  • Cestode

Abstract

Abstract In this study we demonstrate RNA interference mediated knock-down of target gene expression in Echinococcus multilocularis primary cells on both the transcriptional and translational level. In addition, we report on an improved method for generating E. multilocularis primary cell mini-aggregates from in vitro cultivated metacestode vesicles, and on the cultivation of small numbers of small interfering RNA-transfected cells in vitro over an extended period of time. This allows assessments on the effects of RNA interference performed on Echinococcus primary cells with regard to growth, proliferation, differentiation of the parasite and the formation of novel metacestode vesicles in vitro.

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