Affordable Access

Publisher Website

Preparative chromatography of proteins:Design calculation procedure for gradient and stepwise elution

Authors
Journal
Journal of Chromatography A
0021-9673
Publisher
Elsevier
Publication Date
Volume
512
Identifiers
DOI: 10.1016/s0021-9673(01)89475-9

Abstract

Abstract A method is presented for determining stepwise elution chromatographic conditions on the basis of the data obtained from linear gradient elution experiments. The separation of crude β-galactosidase by medium-performance (an)ion-exchange chromatography (MPIEC) was chosen as a model separation system. Some factors affecting the purification such as the column dimensions and the residence time were investigated. Large-scale MPIEC was carried out and its performance was compared with that of a small scale-column. The effect of sample loading was also examined.

There are no comments yet on this publication. Be the first to share your thoughts.