Affordable Access

Publisher Website

Prion protein suppresses perturbation of cellular copper homeostasis under oxidative conditions

Authors
Journal
Biochemical and Biophysical Research Communications
0006-291X
Publisher
Elsevier
Publication Date
Volume
313
Issue
4
Identifiers
DOI: 10.1016/j.bbrc.2003.12.020
Keywords
  • Prion Protein
  • Prplp/Dpl
  • Prp-Deficient Cell Line
  • Copper
  • Manganese
Disciplines
  • Biology

Abstract

Abstract Prion protein (PrP) binds copper and exhibits superoxide dismutase-like activity, while the roles of PrP in copper homeostasis remain controversial. Using Zeeman graphite furnace atomic absorption spectroscopy, we quantified copper levels in immortalized PrP gene ( Prnp)-deficient neuronal cells transfected with Prnp and/or Prnd, which encodes PrP-like protein (PrPLP/Dpl), in the presence or absence of oxidative stress induced by serum deprivation. In the presence of serum, copper levels were not significantly affected by the expression of PrP and/or PrPLP/Dpl, whereas serum deprivation induced a decrease in copper levels that was inhibited by PrP but not by PrPLP/Dpl. The inhibitory effect of PrP on the decrease of copper levels was prevented by overexpression of PrPLP/Dpl. These findings indicate that PrP specifically stabilizes copper homeostasis, which is perturbed under oxidative conditions, while PrPLP/Dpl overexpression prevents PrP function in copper homeostasis, suggesting an interaction of PrP and PrPLP/Dpl and distinct functions between PrP and PrPLP/Dpl on metal homeostasis. Taken together, these results strongly suggest that PrP, in addition to its antioxidant properties, plays a role in stabilizing cellular copper homeostasis under oxidative conditions.

There are no comments yet on this publication. Be the first to share your thoughts.

Statistics

Seen <100 times
0 Comments

More articles like this

Prion protein suppresses perturbation of cellular...

on Biochemical and Biophysical Re... Jan 23, 2004

Cellular prion protein function in copper homeosta...

on Journal of Neurochemistry August 2003

Prion protein expression and functional importance...

on Antioxidants & Redox Signaling Feb 01, 2013

Copper(II) ion binding to cellular prion protein.

on Journal of chemical informatio... February 2008
More articles like this..