Abstract The protective effect of naringin on deltamethrin poisoning in human erythrocyte was studied using an in vitro model. Hemolysis, percentage met-hemoglobin, lipid peroxidation, glutathione, antioxidant enzymes and erythrocyte ghost protein pattern were assessed to investigate the effect of naringin. Erythrocytes at a hematocrit of 10% were incubated with 500ppm of deltamethrin and/or 0.1M naringin under physiological conditions of temperature and pH for 2h. Deltamethrin significantly increased the percentage of hemolysis and met-hemoglobin in human erythrocytes as compared to the control erythrocytes and naringin significantly (P<0.05) inhibited the percentage of hemolysis and met-hemoglobin. The levels of lipid peroxides and conjugated diene increased whereas the level of glutathione decreased significantly (P<0.05) by deltamethrin-incubated erythrocytes. Naringin significantly inhibited the formation of lipid peroxides and conjugated diene while increased the glutathione level in erythrocytes incubated with deltamethrin. The activity of antioxidant enzymes and non-enzymic antioxidants were decreased in erythrocytes incubated with deltamethrin whereas naringin improved the activities of these antioxidant and non-enzymic antioxidants. SDS–PAGE of erythrocyte ghost protein pattern showed an alteration in the protein bands by deltamethrin poisoning but naringin significantly inhibited the alteration in protein profile. The present study divulges that naringin can reduce the abnormalities of deltamethrin poisoning by ameliorating oxidative stress. This finding raises the possibility that naringin may provide protection from pesticide poisoning.