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International workshop on immune assay of nuclear antigens relevant to carcinogenesis and chemotherapy

British Journal of Cancer
Nature Publishing Group
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Br. J. (ancer (1 98X2) 46, 304 INTERNATIONAL WORKSHOP ON IMMUNE ASSAY OF NUCLEAR ANTIGENS RELEVANT TO CARCINOGENESIS AND CHEMOTHERAPY SPONSORED BY BRITISH ASSOCIATION FOR CANCER RESEARCH AND CANCER RESEARCH CAMPAIGN Held at the Paterson Laboratories, Chri,stie Hospital an(l Ilolt Radlium In1stitute, M1anchester J120 9BX Convened bv J. M. BOYLE AND R. SAFFHILIL 26- 27 April 1982 IN RECENT YEARS immunological methods have been applied to elucidating the structure and molecular biology of chromatin, and quantifying carcinogen adducts formed in DNA. Such studies have increased the limits of detection pre- viously available and have opened up new avenues for the study of the distribution of adducts in chromatin and within cel- lular subpopulations. These achievements have accrued from the application of sensitive immunoassays, using polyclonal and now, with increasing importance, monoclonal antibodies. These methods were the subject of a recent International Workshop held at the Paterson Labora- tories. Single-stranded nucleic acids and nucleo- sides modified by carcinogenic agents, when conjugated either electrostatically or covalently with carrier proteins such as bovine serum albumin or haemocyanin, are able to elicit an antibody response. Such conjugates have been used to pro- duce both polyclonal and monoclonal antibodies specific for DNA damage by both physical and chemical agents. Many antibodies have now been characterized and used to quantify carcinogen-DNA adducts, by radioimmunoassay (RIA), enzyme-linked immunosorbent assav (ELISA), ultrasensitive enzyme radio- immune assay (USERIA) or its fluoro- metric equivalent, using 4-methylumbel- liferyl phosphate. Antibodies were described that are specific for thymine glycol produced in DNA irradiated with ionizing radiation or UV (Erlanger), for thymine dimers which are the major UV photoproduct in DNA (Strickland) and for DNA exposed to UV' radiation in the presence of 8-methoxy- psoralen (Zarebska). The exquisite speci- ficity possibl

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