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NMR differentiation of the internal and external phospholipid membrane surfaces using paramagnetic Mn2+and Eu3+ions

Authors
Journal
Chemistry and Physics of Lipids
0009-3084
Publisher
Elsevier
Publication Date
Volume
6
Issue
4
Identifiers
DOI: 10.1016/0009-3084(71)90046-6

Abstract

Abstract The effect of paramagnetic Mn 2+ and Eu 3+ ions on the NMR spectra of sonicated lecithin dispersions in water and benzene has been investigated. In aqueous dispersions containing paramagnetic ions the N + (CH 3) 3 signal of the lecithin molecules in the lipid bilayer consists of two components, one broadened by Mn 2+ and shifted to high field by Eu 3+ must correspond to molecules in the external surface in contact with the bulk salt solution, and the other corresponding to internal lecithin molecules in contact with the ion free internal aqueous phase. Thus the external and internal surfaces of a lecithin containing membrane can be discriminated. The rates of exchange of the paramagnetic ions, of the lecithin and the water molecules between micelles and/or between micelles and external medium have been evaluated.

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