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Preparation of anti-melatonin antibodies and antigenic properties of the molecule

Authors
Journal
Journal of Immunological Methods
0022-1759
Publisher
Elsevier
Publication Date
Volume
32
Issue
4
Identifiers
DOI: 10.1016/0022-1759(80)90026-5

Abstract

Abstract Anti-melatonin antibodies of high titer and good sensitivity were obtained by coupling melatonin to bovine serum albumin with formaldehyde and by immunizing rabbits with multifocal intra-dermal injections. A systematic study of cross-reactions with 14 compounds (indole, aromatic and imidazole derivatives) showed that the antibody had a high specificity for melatonin, low reactivities with 6-hydroxymelatonin and N-acetylserotonin and no detectable reactivity with 12 other derivatives. The roles of the indole nucleus and the side chain in the determination of the antigenic properties of the molecule are discussed.

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