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RecQ helicase, in concert with RecA and SSB proteins, initiates and disrupts DNA recombination.

Authors
Type
Published Article
Journal
Genes & Development
0890-9369
Publisher
Cold Spring Harbor Laboratory Press
Volume
12
Issue
8
Pages
1134–1144
Source
Kowalczykowski Lab

Abstract

RecQ helicase is important to homologous recombination and DNA repair in Escherichia coli. We demonstrate that RecQ helicase, in conjunction with RecA and SSB proteins, can initiate recombination events in vitro. In addition, RecQ protein is capable of unwinding a wide variety of DNA substrates, including joint molecules formed by RecA protein. These data are consistent with RecQ helicase assuming two roles in the cell; it can be (1) an initiator of homologous recombination, or (2) a disrupter of joint molecules formed by aberrant recombination. These findings also shed light on the function of the eukaryotic homologs of RecQ helicase, the Sgs1, Blm, and Wrn helicases.

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