Abstract The relation of environmental stress and plant growth becomes of great interest in agriculture produce. To facilitate the determination of the optimized growth conditions for the Chrysanthemum callus, the uniform design (UD) method is employed. With the aid of the stepwise regression analysis method, the impacts of sound wave stimulation on the development of callus can be elucidated with a limited number of experiments. Guided by the UD method, 12 sets of represented treatment points from the studied experimental domain, incorporating all three variables, namely, the sound wave frequency, strength and loading time are selected to perform the experiment. The response data collected from each of the selected 12 experimental points are then regressed with the studied growth conditions by using the spss software. Acceptable regression equations are obtained in all cases studied. Based on the predictions of the regression equations, several optimized growth of callus targeted at the sound wave frequency, strength and loading time, respectively, are determined. We conclude that sound wave stimulation significantly affects the product of Chrysanthemum callus, the optimum formulation for the relation of the sound wave stimulus and callus growth could be predicted.