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The transcription factor Egr-1 is a regulator of the humanTopBP1gene

Authors
Journal
Gene
0378-1119
Publisher
Elsevier
Publication Date
Volume
380
Issue
2
Identifiers
DOI: 10.1016/j.gene.2006.05.025
Keywords
  • E2F
  • Dna Damage
  • Dna Replication
  • Uv Stress
Disciplines
  • Biology

Abstract

Abstract The human topoisomerase IIβ binding protein 1 (TopBP1) has been reported to be involved in DNA replication, in DNA damage checkpoints and in apoptosis. Detailed analysis of the TopBP1 promoter revealed that the early growth response protein-1 (Egr-1) induces this promoter. Binding of Egr-1 to the TopBP1 promoter was determined to region − 50 to − 18 using EMSA and ChIP technology. Furthermore, deletion of the E2F transcription factor binding sites or mutation of the Egr-1 transcription factor binding sites lead to reduced stimulation of the TopBP1 promoter by Egr-1. These data indicate a cooperative regulation of the TopBP1 promoter by Egr-1 and E2F.

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