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Alteration of human lymphocyte triosephosphate isomerase isozymes during blastogenesis

Authors
Journal
Biochemical and Biophysical Research Communications
0006-291X
Publisher
Elsevier
Publication Date
Volume
65
Issue
4
Identifiers
DOI: 10.1016/s0006-291x(75)80367-6

Abstract

Summary Human lymphocytes contain a single isozyme of triosephosphate isomerase (pI=5.6). However, when induced to undergo blastogenesis by culturing in the presense of mitogens, a more acidic isozyme (pI=5.2) is also produced concomitant with DNA replication. Culturing of lymphocytes in the absence of mitogens or incubation of homogenates from lymphoblasts with fresh lymphocyte extracts does not generate the new isozyme. This system provides, for the first time, an in vivo method for studying the molecular basis and factors regulating the expression of the different isozymes of human triosephosphate isomerase.

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