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Dysfunctional role of elevated TIGIT expression on T cells in oral squamous cell carcinoma patients.

Authors
  • Liu, Xiangqi1
  • Li, Qunxing2
  • Zhou, Ying2
  • He, Xinlin2
  • Fang, Juan2
  • Lu, Huanzi2
  • Wang, Xi2
  • Wang, Dikan2
  • Ma, Da3
  • Cheng, Bin2
  • Liao, Guiqing1
  • Wang, Zhi2
  • 1 Department of Oral and Maxillofacial Surgery, Hospital of Stomatology, Guanghua School of Stomatology, Guangdong Provincial Key Laboratory of Stomatology, Sun Yat-sen University, Guangzhou, China. , (China)
  • 2 Department of Oral Medicine, Hospital of Stomatology, Guanghua School of Stomatology, Guangdong Provincial Key Laboratory of Stomatology, Sun Yat-sen University, Guangzhou, China. , (China)
  • 3 Department of Prosthodontics, Hospital of Stomatology, Guanghua School of Stomatology, Guangdong Provincial Key Laboratory of Stomatology, Sun Yat-sen University, Guangzhou, China. , (China)
Type
Published Article
Journal
Oral diseases
Publication Date
Oct 01, 2021
Volume
27
Issue
7
Pages
1667–1677
Identifiers
DOI: 10.1111/odi.13703
PMID: 33125794
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

This study was aimed to analyze the role of T-cell immunoreceptor with immunoglobulin and tyrosine-based inhibitory motif domains (TIGIT) expression on T cells in patients with oral squamous cell carcinoma (OSCC). Peripheral blood mononuclear cells (PBMC) and tumor-infiltrating lymphocytes (TILs) were collected from OSCC patients. The correlation between TIGIT expression and clinicopathologic features was analyzed by chi-square test. Phenotypic and functional study of TIGIT+ T cells were performed by flow cytometry. TIGIT was highly expressed on T cells from PBMC and TILs. High expression of TIGIT on CD4+ T cells (19.0%) and CD8+ T cells (35.9%) was also associated with higher T stage and nodal invasion. Moreover, TIGIT+ CD4+ and TIGIT+ CD8+ T cells sorted from OSCC patients showed a dysfunctional phenotype (low cell proliferation and low secretion of IL-2, TNF-α and IFN-γ), and TIGIT+ CD4+ T cells exhibited inhibitory function (high expression of Foxp3 and high amounts of IL-10). Importantly, TIGIT blockade can enhance the proliferation ability and effective cytokine production (IL-2, TNF-α, and IFN-γ) of CD4+ and CD8+ T cells from OSCC patients in vitro. TIGIT-expressing T cells exhibit a lower effector cytokine-releasing phenotype in OSCC patients. © 2020 Wiley Periodicals LLC.

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