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Dynamics of volatilomes emitted during cross-talking of plant-growth-promoting bacteria and the phytopathogen, Fusarium solani

Authors
  • Ana, A. Gutiérrez-Santa1
  • Carrillo-Cerda, H. A.1
  • Rodriguez-Campos, J.2
  • Velázquez-Fernández, J. B.3
  • Patrón-Soberano, O. A.4
  • Contreras-Ramos, S. M.1
  • 1 Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco A.C. (CIATEJ), Av. Normalistas No. 800, Col. Colinas de La Normal, Guadalajara, Jalisco, 44270, Mexico , Guadalajara (Mexico)
  • 2 CIATEJ, Av. Normalistas No. 800, Col. Colinas de La Normal, Guadalajara, Jalisco, 44270, Mexico , Guadalajara (Mexico)
  • 3 Catedra-Conacyt assigned to Unidad de Tecnología Ambiental at CIATEJ, Guadalaja, Jalisco, Mexico , Guadalaja (Mexico)
  • 4 Instituto Potosino de Investigación Científica Y Tecnológica A.C. (IPICYT), Camino a la Presa San José 2055, Lomas 4ª. Sección, San Luis Potosí, San Luis Potosí, 78216, Mexico , San Luis Potosí (Mexico)
Type
Published Article
Journal
World Journal of Microbiology and Biotechnology
Publisher
Springer Netherlands
Publication Date
Sep 14, 2020
Volume
36
Issue
10
Identifiers
DOI: 10.1007/s11274-020-02928-w
Source
Springer Nature
Keywords
License
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Abstract

AbstractThe dynamics of volatilomes emitted during the interaction between plant-growth-promoting bacteria (PGPB) and the phytopathogen Fusarium solani were evaluated for 5 days. The first screening was done to evaluate the antagonist activity of volatile compounds emitted by PGPB against F. solani. Volatilomes from 11 PGPB were determined individually and together with F. solani by using solid-phase microextraction coupled to gas-chromatography–mass spectrometry. Isolates of PGPB belonged to the Bacillus genus and inhibited from 18 to 24% the fungal mycelium growth. The isolates also induced morphological alterations of fungal hyphae, like small globular vesicles and the formation of chlamydospores, suggesting a stress mechanism response by the fungus. Volatilome profile showed 49 different compounds that appeared in the bacterial–fungal interaction, such as ketones, sesquiterpenes, monoterpenoids, alkanes, alkenes, carboxylic acids, and fatty acids. Some ketones and alcohols were detected in high abundance only in the interaction PGPB-fungus at 3 and 5 days. Bacillus circulans A19, Bacillus amyloliquefaciens A21, and Bacillus wiedmannii S18 shared a group of emitted alcohols and ketones when they were exposed to F. solani. F. solani produced its own volatilome profile, with the presence of sesquiterpenes, such as α-cubebene and caryophyllene, which increased significantly in co-incubation with the tested bacteria, suggesting chemical communication between them.Graphic abstract

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