Dynamics of success and failure in phage and antibiotic therapy in experimental infections

Affordable Access

Dynamics of success and failure in phage and antibiotic therapy in experimental infections

Publisher
BioMed Central
Publication Date
Nov 26, 2002
Source
PMC
Keywords
Disciplines
  • Biology
  • Medicine
License
Unknown

Abstract

1471-2180-2-35.fm ral ss BioMed CentBMC Microbiology Open AcceBMC Microbiology 2002, 2 xResearch article Dynamics of success and failure in phage and antibiotic therapy in experimental infections J J Bull1, Bruce R Levin*2, Terry DeRouin2, Nina Walker2 and Craig A Bloch3 Address: 1Section of Integrative Biology and Institute for Cellular and Molecular Biology, University of Texas, Austin, TX 78712-1023, USA, 2Department of Biology, Emory University, Atlanta, GA 30322, USA and 3Department of Pediatrics, University of Michigan Medical School, Ann Arbor, MI 48104, USA E-mail: J J Bull - [email protected]; Bruce R Levin* - [email protected]; Terry DeRouin - [email protected]; Nina Walker - [email protected]; Craig A Bloch - [email protected] *Corresponding author Abstract Background: In 1982 Smith and Huggins showed that bacteriophages could be at least as effective as antibiotics in preventing mortality from experimental infections with a capsulated E. coli (K1) in mice. Phages that required the K1 capsule for infection were more effective than phages that did not require this capsule, but the efficacies of phages and antibiotics in preventing mortality both declined with time between infection and treatment, becoming virtually ineffective within 16 hours. Results: We develop quantitative microbiological procedures that (1) explore the in vivo processes responsible for the efficacy of phage and antibiotic treatment protocols in experimental infections (the Resistance Competition Assay, or RCA), and (2) survey the therapeutic potential of phages in vitro (the Phage Replication Assay or PRA). We illustrate the application and utility of these methods in a repetition of Smith and Huggins' experiments, using the E. coli K1 mouse thigh infection model, and applying treatments of phages or streptomycin. Conclusions: 1) The Smith and Huggins phage and antibiotic therapy results are quantitatively and qualitatively robust. (2) Our RCA values reflect the microbi

Report this publication

Statistics

Seen <100 times