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Dynamics of neutrophil infiltration during cutaneous wound healing and infection using fluorescence imaging.

Authors
  • Kim, Min-Ho
  • Liu, Wei
  • Borjesson, Dori L
  • Curry, Fitz-Roy E
  • Miller, Lloyd S
  • Cheung, Ambrose L
  • Liu, Fu-Tong
  • Isseroff, R Rivkah
  • Simon, Scott I
Type
Published Article
Journal
Journal of Investigative Dermatology
Publisher
Elsevier
Publication Date
Jul 01, 2008
Volume
128
Issue
7
Pages
1812–1820
Identifiers
DOI: 10.1038/sj.jid.5701223
PMID: 18185533
Source
Medline
License
Unknown

Abstract

Neutrophil influx is an early inflammatory response that is essential for the clearance of bacteria and cellular debris during cutaneous wounding. A non-invasive real-time fluorescence imaging technique was developed to examine the kinetics of enhanced green fluorescence protein-polymorphonuclear leukocyte (EGFP-PMN) influx within a wound. We hypothesized that infection or systemic availability would directly regulate the dynamics of EGFP-PMN recruitment and the efficiency of wound closure. Neutrophil recruitment increased dramatically over the first 24 hours from 10(6) at 4 hours up to a maximum of 5 x 10(6) EGFP-PMNs at 18 hours. A high rate of EGFP-PMN turnover was evidenced by approximately 80% decrease in EGFP signal within 6 hours. In response to wound colonization by Staphylococcus aureus or injection of GM-CSF, systemic PMNs increased twofold above saline control. This correlated with an increase in EGFP-PMN recruitment up to approximately 10(7) within the wound. Despite this effect by these distinct inflammatory drivers, wound closure occurred at a rate similar to the saline-treated control group. In summary, a non-invasive fluorescence-based imaging approach combined with genetic labeling of neutrophils provides a dynamic inner view of inflammation and the kinetics of neutrophil infiltration into the wounded skin over extended durations.

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