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Dynamic nucleosome remodeling mediated by YY1 underlies early mouse development.

Authors
  • Sakamoto, Mizuki1
  • Abe, Shusaku2
  • Miki, Yuka2
  • Miyanari, Yusuke3
  • Sasaki, Hiroyuki2
  • Ishiuchi, Takashi4
  • 1 Faculty of Life and Environmental Sciences, University of Yamanashi, Yamanashi 400-8510, Japan. , (Japan)
  • 2 Division of Epigenomics and Development, Medical Institute of Bioregulation, Kyushu University, Fukuoka 812-8582, Japan. , (Japan)
  • 3 NanoLSI, Cancer Research Institute, Kanazawa University, Kanazawa 920-1192, Japan. , (Japan)
  • 4 Faculty of Life and Environmental Sciences, University of Yamanashi, Yamanashi 400-8510, Japan; [email protected]. , (Japan)
Type
Published Article
Journal
Genes & development
Publication Date
Jul 01, 2023
Volume
37
Issue
13-14
Pages
590–604
Identifiers
DOI: 10.1101/gad.350376.122
PMID: 37532472
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Nucleosome positioning can alter the accessibility of DNA-binding proteins to their cognate DNA elements, and thus its precise control is essential for cell identity and function. Mammalian preimplantation embryos undergo temporal changes in gene expression and cell potency, suggesting the involvement of dynamic epigenetic control during this developmental phase. However, the dynamics of nucleosome organization during early development are poorly understood. In this study, using a low-input MNase-seq method, we show that nucleosome positioning is globally obscure in zygotes but becomes well defined during subsequent development. Down-regulation of the chromatin assembly in embryonic stem cells can partially reverse nucleosome organization into a zygote-like pattern, suggesting a possible link between the chromatin assembly pathway and fuzzy nucleosomes in zygotes. We also reveal that YY1, a zinc finger-containing transcription factor expressed upon zygotic genome activation, regulates the de novo formation of well-positioned nucleosome arrays at the regulatory elements through identifying YY1-binding sites in eight-cell embryos. The YY1-binding regions acquire H3K27ac enrichment around the eight-cell and morula stages, and YY1 depletion impairs the morula-to-blastocyst transition. Thus, our study delineates the remodeling of nucleosome organization and its underlying mechanism during early mouse development. © 2023 Sakamoto et al.; Published by Cold Spring Harbor Laboratory Press.

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